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小型垂直电泳

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Mini-PROTEAN 3 电泳系统

Mini-PROTEAN 3 电泳系统

图片
序列号:165-3301

【介绍】

功能灵活的Mini–PROTEAN 3 电泳槽既可使用 Ready Gel 预制胶,也可使用手灌胶。 它是目前开发的相关产品中最方便的手灌胶操作系 统。Mini–PROTEAN 3 电泳系统省去了冗长的组装程 序,简化了灌胶和电泳过程。Mini–PROTEAN 3 电泳 槽可用于:

  • 一小时内完成小型胶电泳
  • 一天内完成双向凝胶电泳
  • 用于探索型的课题研究
  • 筛选新样品
  • 评估样品制备条件
制胶优点:
  • 封边垫条永久地固定在长玻板上,保证玻板精确对齐,防止漏胶
  • 凸轮卡锁的制胶框操作简单*,在任何平面上都能精确对齐玻板
  • 特殊的塑料电泳梳*不会抑制凝胶聚合反应,制胶过程中,内置的脊可避免的空气接触,保证均一的凝胶聚合
  • 标有厚度和孔数的玻板和电泳梳便于识别
  • 平行排列的灌胶架*能同时看到正在灌制的两块凝胶,弹簧杠杆设计使得软橡胶衬垫产生良好的密封性
  • 含封边垫条的长玻板加厚,使得玻板不宜破碎。
上样和电泳的创新性
  • 专利的上样引导装置**,防止泳道的遗漏上样或重复上样
  • 电泳槽可同时进行2 块凝胶电泳,或使用塑模档板进行1 块凝胶电泳
模块式设计

模块式设计可使Mini–PROTEAN 3 电泳槽有多种用途。每个模块可与Mini–PROTEAN 3 的缓冲液槽和盖匹配, 形成一个完整的电泳槽。有以下4 个模块可选择:

  • Mini–PROTEAN 3 电泳槽,适合手灌胶或预制PAGE 和SDS–PAGE 胶
  • 小型Trans–Blot 电泳槽,适合western 印迹杂交;
  • 422 型电洗脱仪,用于电洗脱样品条带或整个泳道以进行探索研究;
  • Mini–PROTEAN 2–D 电泳槽,用于双向应用中的第一向IEF 管式凝胶;

更多信息,请索取资料# 2378。

* US 专利6,162,342
** US 专利5,656,145

简易的装配流程。永久固定的封边垫条保证了胶板正确对位并防止漏胶。只需在任何平面上将制胶框和玻板组装起来,就完成了胶板对 齐。制胶架上的弹簧杠杆可在橡胶密封衬垫上形成良好的密封性。

 

将凝胶夹(gel cassettes)装配好,转移到固夹框clamping frame(插入固夹框时,轻柔地把凝胶夹固定于适当位置),运用外表柔和的凸 轮卡锁将凝胶固定在密封垫上。这样凝胶就可以进行电泳了。

 

Mini–PROTEAN 3 电泳梳的每孔最大样品体积

    电泳梳厚度
孔数或孔类型 孔宽度 0.5 mm 0.75 mm 1.0 mm 1.5 mm
5 12.70 mm 70 µl 105 µl 160 µl
9 5.08 mm 33 µl 44 µl 66 µl
10 5.08 mm 22 µl 33 µl 44 µl 66 µl
15 3.35 mm 13 µl 20 µl 26 µl 40 µl
IPG 76.20 mm 420 µl 730 µl
Prep/2–D  
Reference well 3.10 mm 13 µl 17 µl 30 µl
Sample well 71.70 mm 310 µl 400 µl 680 µl

将Mini–PROTEAN II 电泳槽升级到Mini–PROTEAN 3 电泳槽

只需3 个简单的步骤就可将Mini–PROTEAN II电泳槽改装成Mini–PROTEAN 3电泳槽:

  1. 保留Mini–PROTEAN II 的槽和盖。
  2. 定购Mini–PROTEAN II 升级组合套件(货号#165–3375)。
  3. 定购所需的制胶模块。
选购适合您的电泳系统

可从预设配置中选择电泳系统,定购货号为#165–3301(10 孔、0.75 mm)或165–3322 (10 孔、1.0 mm)。 如要装配你自己的电泳系统,你可以购买Mini–PROTEAN 3 电泳模块(货号#165–3302)并从订货信息中另外选 择一种制胶模块。如果你只使用ReadyGel 预制胶,Mini–PROTEAN 3 电泳模块能满足你的需要。

【技术指标】

凝胶数 1 或2
预制胶 Ready Gel 预制胶
手灌胶 Mini-PROTEAN 3 长玻板灌制
玻板尺寸(W x L)  
  短板 10.1 x 7.3 cm
  间隔板 10.1 x 8.2 cm
凝胶大小(W x L) 手灌胶:8.3 x 7.3 cm; 预制胶: 8.6 x 6.8 cm
典型上层缓冲液体积 120 ml
典型下层缓冲液体积 180 ml
典型SDS-PAGE电泳时间 45 min ( 200 V 恒压)
建议电源 PowerPac Basic 或 PowerPac HC
大小(W x L x H) 12 x 16 x 18 cm
重量 2.0 kg

【订货信息】

目录 # 描述
Mini-PROTEAN 3 Cell
165-3301 Mini-PROTEAN 3 Electrophoresis Cell, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam)
165-3302 Mini-PROTEAN 3 Electrophoresis Module, for Ready Gel precast gel applications, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam
Systems
165-2959 Mini-PROTEAN 3 Electrophoresis Cell/Mini Trans-Blot Electrophoretic Transfer Cell, 10-well, 0.75 mm thickness; complete system includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer chamber, lid with cables, instructions
165-3317 Mini-PROTEAN 3 Electrophoresis Cell and Mini Trans-Blot Module, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module
165-3318 Mini-PROTEAN 3 Electrophoresis Module and Mini Trans-Blot Module, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam, Mini Trans-Blot module
165-3319 Mini-PROTEAN 3 Cell and PowerPac Basic Power Supply, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 100–120/220–240 V power supply includes power cord, instructions
165-3321 Mini-PROTEAN 3 Cell and Single-Row AnyGel Stand, includes 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), single-row AnyGel stand holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions
165-3323 Mini-PROTEAN 3 Electrophoresis Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply
165-3324 Mini-PROTEAN 3 Electrophoresis Module, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply
170-3989 Mini Trans-Blot Cell and PowerPac Basic Power Supply, includes 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer tank, lid with cables, power supply, power cord, instructions
Accessories
165-3320 Gel Releasers, 5
165-4131 AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions
165-4132 Replacement Clamps, 2
165-5131 AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 ReadyGel mini gels; includes instructions
Casting Modules
165-3325 Casting Module, 10-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3326 Casting Module, 15-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3327 Casting Module, 5-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3328 Casting Module, 9-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3329 Casting Module, 10-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3330 Casting Module, 15-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3331 Casting Module, prep/2-D well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3332 Casting Module, 5-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3333 Casting Module, 9-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3334 Casting Module, 10-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3335 Casting Module, 15-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3336 Casting Module, prep/2-D well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3337 Casting Module, IPG well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3338 Casting Module, 5-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3339 Casting Module, 9-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3340 Casting Module, 10-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3341 Casting Module, 15-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide
165-3342 Casting Module, prep/2-D well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates
165-3343 Casting Module, IPG well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates

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